Effects of different current field condition on the symmetrical fusion of cotyledonary protoplasts between Cucummis sativus L. and Cucurbita ficifolia Bouche

The studies of symmetrical fusion of protoplasts isolated from Cuixiu mini cucumber (Cucummis sativus L. ) and fig-baf gourd (Cucurbita ficifolia Bouche) were carried out. The results indicated that the conditions could lead to the best effect of cell fusion were using 0.5 mol·L-1 mannitol as fusional solution and setting the alternating current field intensity at 25 V·cm-1, the alternating current frequency at 2000 kHz, the direct current intensity at 200 V·cm-1, the direct current pulse width at 40μs and 3 direct pulse times. After cultured in shallow-layer liquid medium, large numbers of callus were regenerated. The genetic identification of callus and the plant regeneration were also evaluated.