- 中文核心期刊
- CSCD来源期刊
- 中国科技核心期刊
- CA、CABI、ZR收录期刊
| Citation: |
LIN Y Q，LI W，LIANG Q Z，et al. Epidemiological survey of waterfowl circovirus and molecular characteristics of the viral genomes in natural co-infected case in goose[J]. Fujian Journal of Agricultural Sciences，2025，X（X） ：1−8.
|
To investigate the prevalence of waterfowl circovirus (WFCV) (including goose circovirus (GoCV) and duck circovirus (DuCV)) in the goose flocks, and to explore the molecular characteristics of the GoCV and DuCV genomes in the first natural co-infection case in goose.
A total of 153 goose tissue samples were collected from goose flocks in six provinces, including Fujian, Guangxi, and Anhui, during 2023-2024. The GoCV and DuCV in the goose samples were detected by using a tri-primer PCR method, and the whole genome sequences of GoCV and DuCV were determined and analysed in a natural co-infection case in a goose.
Fifty-three WFCV positive samples were identified from 153 goose tissue samples, with a positivity rate of 34.6%. Of the WFCV positive samples found, 47 were single positive for GoCV, 5 were single positive for DuCV and 1 sample (AH2367) from Anhui Province was found to be co-infected with GoCV and DuCV. The whole genome sequence of GoCV (Go-AH2367 strain) in the co-infection case was 1821 nt in length, with a homology of 82.9%-99.0% with the GoCV sequences, and 67.0%-70.9% with the DuCV sequences deposited in the GenBank database. The DuCV (Du-AH2367 strain) genome in the co-infection case was 1992 nt in length, with a homology of 84.4%-99.7% with the DuCV sequences, and 66.1%-68.8% with GoCV genome sequences deposited in the GenBank database. The amino acid sequences of the two major functional proteins of these two co-infected viruses showed only sporadic site variation, with no gene recombination, site deletion or insertion, and the major functional motifs in the two proteins were highly conserved. Phylogenetic analysis showed that the Go-AH2367 strain was clusted into the same evolutionary branch of the genotype 1 GoCV isolates, and the Du-AH2367 strain was most closely related to genotype 1 DuCV isolates and clusted into the same evolutionary branch.
WFCV infection in goose flocks is relatively severe, and were dominated by GoCV infections, accompanied by cross-species infection with DuCV and co-infection with GoCV and DuCV, presenting an epidemic of viral ecological diversity. The genomes of the two viral strains in the co-infected samples were genetically stable, with no obvious genetic variation such as gene deletion or recombination.
| [1] |
TODD D,WESTON J H,SOIKE D,et al. Genome sequence determinations and analyses of novel circoviruses from goose and pigeon[J]. Virology,2001,286(2) :354−362. DOI: 10.1006/viro.2001.0985
|
| [2] |
SOIKE D,KOHLER B,ALBRECHT K. A circovirus-like infection in geese related to a runting syndrome[J]. Avian Pathology,1999,28(2) :199−202. DOI: 10.1080/03079459994939
|
| [3] |
HATTERMANN K,SCHMITT C,SOIKE D,et al. Cloning and sequencing of duck circovirus (DuCV) [J]. Archives of Virology,2003,148(12) :2471−2480. DOI: 10.1007/s00705-003-0181-y
|
| [4] |
STENZEL T,FARKAS K,VARSANI A. Genome sequence of a diverse goose circovirus recovered from greylag goose[J]. Genome Announcements,2015,3(4) :e00767−15.
|
| [5] |
KOZDRUŃ W,WOŹNIAKOWSKI G,SAMOREK-SALAMONOWICZ E,et al. Viral infections in goose flocks in Poland[J]. Polish Journal of Veterinary Sciences,2012,15(3) :525−530. DOI: 10.2478/v10181-012-0080-9
|
| [6] |
万春和,施少华,程龙飞,等. 朗德鹅圆环病毒全基因组序列测定和遗传演化分析[J]. 中国动物传染病学报,2010,18(4) :6−12. DOI: 10.3969/j.issn.1674-6422.2010.04.002
WAN C H,SHI S H,CHENG L F,et al. Genomic cloning and sequence analysis of circovirus from Landes geese[J]. Chinese Journal of Animal Infectious Diseases,2010,18(4) :6−12. (in Chinese) DOI: 10.3969/j.issn.1674-6422.2010.04.002
|
| [7] |
余旭平,郑新添,何世成,等. 鹅圆环病毒浙江永康株全基因组的克隆及序列分析[J]. 微生物学报,2005,45(6) :860−864. DOI: 10.3321/j.issn:0001-6209.2005.06.009
YU X P,ZHENG X T,HE S C,et al. Cloning and analysis of the complete genome of a Goose circovirus from Yongkang Zhejiang[J]. Acta Microbiologica Sinica,2005,45(6) :860−864. (in Chinese) DOI: 10.3321/j.issn:0001-6209.2005.06.009
|
| [8] |
SCOTT A N J,BECKETT A,SMYTH J A,et al. Serological diagnosis of goose circovirus infections[J]. Avian Pathology,2006,35(6) :495−499. DOI: 10.1080/03079450601087841
|
| [9] |
CHEN C L,CHANG P C,LEE M S,et al. Nucleotide sequences of goose circovirus isolated in Taiwan[J]. Avian Pathology,2003,32(2) :165−171. DOI: 10.1080/0307945021000071614
|
| [10] |
CHEN J D,WANG H,PEI H,et al. The prevalence,coinfection,and evolutionary and molecular characteristics of prevalent goose circovirus in Guangdong,China[J]. Avian Diseases,2021,65(4) :559−571.
|
| [11] |
BANDA A,GALLOWAY-HASKINS R I,SANDHU T S,et al. Genetic analysis of a duck circovirus detected in commercial Pekin ducks in New York[J]. Avian Diseases,2007,51(1) :90−95. DOI: 10.1637/0005-2086(2007)051[0090:GAOADC]2.0.CO;2
|
| [12] |
FRINGUELLI E,SCOTT A N J,BECKETT A,et al. Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests[J]. Avian Pathology,2005,34(6) :495−500.
|
| [13] |
CHEN C L,WANG P X,LEE M S,et al. Development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus[J]. Avian Diseases,2006,50(1) :92−95. DOI: 10.1637/7435-090705R1.1
|
| [14] |
LEI X N,WANG A P,ZHU S Y,et al. From obscurity to urgency: A comprehensive analysis of the rising threat of duck circovirus[J]. Veterinary Research,2024,55(1) :12. DOI: 10.1186/s13567-024-01265-2
|
| [15] |
陈济铛,陈冈,牛森,等. 广东鹅源圆环病毒分子流行病学调查与毒株遗传进化分析[J]. 中国家禽,2020,42(4) :97−102.
CHEN J D,CHEN G,NIU S,et al. Epidemiological investigation and genome analysis of goose circovirus in Guangdong of China[J]. China Poultry,2020,42(4) :97−102. (in Chinese)
|
| [16] |
LIU H,LI L X,SUN W C,et al. Molecular survey of duck circovirus infection in poultry in southern and southwestern China during 2018 and 2019[J]. BMC Veterinary Research,2020,16(1) :80. DOI: 10.1186/s12917-020-02301-x
|
| [17] |
TRAN G T H,MAI N T,BUI V N,et al. Duck circovirus in northern Vietnam: Genetic characterization and epidemiological analysis[J]. Archives of Virology,2022,167(9) :1871−1877. DOI: 10.1007/s00705-022-05501-y
|
| [18] |
赵光伟,谭雅文,李金凤,等. 重庆地区一例鹅感染鸭圆环病毒病的PCR诊断[J]. 畜禽业,2009,20(6) :66−68. DOI: 10.3969/j.issn.1008-0414.2009.06.042
ZHAO G W,TAN Y W,LI J F,et al. Diagnosis of duck circovirus infected the goose by PCR in Chongqing area[J]. Livestock and Poultry Industry,2009,20(6) :66−68. (in Chinese) DOI: 10.3969/j.issn.1008-0414.2009.06.042
|
| [19] |
SHEHATA A A,GERRY D M,HEENEMANN K,et al. Goose parvovirus and circovirus coinfections in ornamental ducks[J]. Avian Diseases,2016,60(2) :516−522. DOI: 10.1637/11335-112615-Case
|
| [20] |
XU S Q,MAN Y Z,XU X,et al. Genetic heterogeneity of duck circovirus first detected in geese from China[J]. Poultry Science,2024,103(12) :104284. DOI: 10.1016/j.psj.2024.104284
|
| [21] |
XU S Q,MU X H,XU X,et al. First report of goose circovirus identified in ducks from China[J]. Poultry Science,2024,103(8) :103936. DOI: 10.1016/j.psj.2024.103936
|
| [22] |
FU H R,ZHAO M,CHEN S Y,et al. Simultaneous detection and differentiation of DuCV-1 and DuCV-2 by high-resolution melting analysis[J]. Poultry Science,2024,103(4) :103566. DOI: 10.1016/j.psj.2024.103566
|
| [23] |
GUO J,TIAN J,TAN X,et al. Pathological observations of an experimental infection of geese with goose circovirus[J]. Avian Pathology,2011,40(1) :55−61. DOI: 10.1080/03079457.2010.538371
|
| [24] |
万春和,黄瑜. 鹅圆环病毒研究进展[J]. 中国家禽,2015,37(9) :46−48
WAN C H,HUANG Y. Research progress in goose circovirus[J]. China Poultry,2015,37(9) :46−48. (in Chinese)
|
| [25] |
王梦鸽,黄瑜,傅秋玲,等. 鸭圆环病毒和鹅圆环病毒三引物鉴别检测方法的建立[J]. 福建农业学报,2023,38(1) :7−11.
WANG M G,HUANG Y,FU Q L,et al. Establishment of a triple-primer multiplex PCR assay for discriminative detection of duck and goose circoviruses[J]. Fujian Journal of Agricultural Sciences,2023,38(1) :7−11. (in Chinese)
|
| [26] |
CHEN C J,WU Y,LI J W,et al. TBtools-II: A “one for all,all for one” bioinformatics platform for biological big-data mining[J]. Molecular Plant,2023,16(11) :1733−1742. DOI: 10.1016/j.molp.2023.09.010
|
| [27] |
TODD D. Circoviruses: Immunosuppressive threats to avian species: A review[J]. Avian Pathology,2000,29(5) :373−394. DOI: 10.1080/030794500750047126
|
| [28] |
SOIKE D,ALBRECHT K,HATTERMANN K,et al. Novel circovirus in mulard ducks with developmental and feathering disorders[J]. Veterinary Record,2004,154(25) :792−793. DOI: 10.1136/vr.154.25.792
|
| [29] |
黄瑜,万春和,彭春香,等. 鸭圆环病毒感染的临床症状[J]. 中国家禽,2013,35(5) :47−48. DOI: 10.3969/j.issn.1004-6364.2013.05.013
HUANG Y,WAN C H,PENG C X,et al. Clinical symptoms of duck circovirus infection[J]. China Poultry,2013,35(5) :47−48. (in Chinese) DOI: 10.3969/j.issn.1004-6364.2013.05.013
|
| [30] |
傅光华,程龙飞,施少华,等. 鸭圆环病毒全基因组克隆与序列分析[J]. 病毒学报,2008,24(2) :138−143. DOI: 10.3321/j.issn:1000-8721.2008.02.010
FU G H,CHENG L F,SHI S H,et al. Genome cloning and sequence analysis of duck circovirus[J]. Chinese Journal of Virology,2008,24(2) :138−143. (in Chinese) DOI: 10.3321/j.issn:1000-8721.2008.02.010
|
| [1] | ZHOU Yanfang, WANG Xiuzhi, ZHANG Xiaomei, LUO Xiangwen, LIU Zehong, CUI Congcong, MENG Lingqiang, SUN Junling. Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng, Inner Mongolia[J]. Fujian Journal of Agricultural Sciences, 2024, 39(7): 819-825. DOI: 10.19303/j.issn.1008-0384.2024.07.009 |
| [2] | WANG Mengge, HUANG Yu, FU Qiuling, CHENG Longfei, WAN Chunhe, LIU Rongchang, CHEN Hongmei, JIANG Nansong, LIANG Qizhang, SHI Shaohua, LIN Jiansheng, FU Guanghua. Establishment of a Triple-primer Multiplex PCR Assay for Discriminative Detection of Duck and Goose Circoviruses[J]. Fujian Journal of Agricultural Sciences, 2023, 38(1): 7-11. DOI: 10.19303/j.issn.1008-0384.2023.01.002 |
| [3] | ZHENG Nen-zhu, ZHANG Lin-li, LI Li, XIN Qing-wu, MIAO Zhong-wei, ZHU Zhi-ming, HUANG Yi-fan. Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 933-938. DOI: 10.19303/j.issn.1008-0384.2019.08.010 |
| [4] | WU Xiao-mei, YE Mei-feng, WU Fei-long, LIN Dai-yan. Cu and Zn Accumulations in Myriophyllum spicatum for Purification of Pig Farm Biogas Slurry[J]. Fujian Journal of Agricultural Sciences, 2018, 33(11): 1195-1200. DOI: 10.19303/j.issn.1008-0384.2018.11.013 |
| [5] | Li-bin HE, Fang CHEN, Zhi-huang ZHU, Min-ning MA, He-chang ZHANG, Sun-long WU, Chen ZHOU. Molecular Phylogeny Determined by Mitochondrial Genes 16S rRNA, COX1 and Cytb of Eleven Anemonefish Species[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 230-235. DOI: 10.19303/j.issn.1008-0384.2018.03.003 |
| [6] | WANG Cui, XIE Bao-gui, CHEN Liang-jun, KE Ting, KE Huo-zhao. SCAR Molecular Identification of 30 Flammulina velutipes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 966-970. DOI: 10.19303/j.issn.1008-0384.2013.10.007 |
| [7] | WANG Xiao-min, HE Kong-wang, WANG Dong-tian, ZHOU Zhong-tao, MAO Ai-hua, YU Zheng-yu, WANG Wei, NI Yan-xiu, WEN Li-bin. Investigation on Epidemiology of Co-infection of PCV2,P1 and TTV[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 864-868. DOI: 10.19303/j.issn.1008-0384.2013.09.006 |
| [8] | WANG Shao, CHENG Xiao-xia, CHEN Shao-ying, ZHU Xiao-li, CHEN Shi-long, LIN Feng-qiang, LI Zhao-long. Molecular Characteristics Analysis of D-strain Vaccine Virus of Muscovy Duck-origin Goose Parvovirus NS Protein Epitopes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 301-308. DOI: 10.19303/j.issn.1008-0384.2013.04.001 |
| [9] | CHE Yong-liang, SHI Yun-tong, ZHUANG Xiang-sheng, CHEN Shao-ying, WANG Long-bai, WEI Hong, CHEN Ru-jing, WU Xue-min, ZHOU Lun-jiang. Effect of Porcine circovirus Type 2 ORF2 Gene Expression in PcDNA3.1 for Vacination[J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 166-169. |
| [10] | WAN Chun-he, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, HUANG Yu. Nested PCR detection and sequence analysis on Zhejiang duck circovirus[J]. Fujian Journal of Agricultural Sciences, 2009, 24(5): 390-395. |
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: