OsPLGG1 enhanced function of photorespiratory bypass

CUI Lili; CAI Qiuhua; QIU Jieyu; GAO Rongrong; ZHAO Yongchao; WANG Yingheng; PENG Xinxiang; ZHU Guohui; ZHANG Jianfu

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CUI L L, CAI Q H, QIU J Y, et al. OsPLGG1 enhanced function of photorespiratory bypass [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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CUI L L, CAI Q H, QIU J Y, et al. OsPLGG1 enhanced function of photorespiratory bypass [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

CUI L L, CAI Q H, QIU J Y, et al. OsPLGG1 enhanced function of photorespiratory bypass [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

Citation:

CUI L L, CAI Q H, QIU J Y, et al. OsPLGG1 enhanced function of photorespiratory bypass [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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OsPLGG1 enhanced function of photorespiratory bypass

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture and Rural Affairs /Fuzhou branch, National Rice Improvement Center of China/National Engineering Laboratory of Rice/Incubator of Key Laboratory of Crop Germplasm Enhancement and Molecular Breeding between Fujian and Ministry of Sciences and Technology/ Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Research Base of South-China of National Key Laboratory of Hybrid Rice of China, Fuzhou, Fujian　350003, China
2.
College of Life Sciences, South China Agricultural University/State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, , Guangzhou, Guangdong　510642, China

Received Date: 2024-05-24
Rev Recd Date: 2024-07-06

Available Online: 2024-11-06

Abstract

Abstract

Objective The deletion of either OsPLGG1a or OsPLGG1b in the GOC rice engineering optimizes the photorespiration pathway, thereby enhancing rice photosynthesis efficiency and optimizing photorespiration metabolic engineering. Method The knockout vectors osplgg1a-Cas9 or osplgg1b-Cas9 were constructed and transformed into GOC rice, respectively, to obtain GOC transgenic homozygous lines with deletion of OsPLGG1a or OsPLGG1b, and the photosynthesis rate was measured. Result Transgenic homozygous lines with the GOC rice of OsPLGG1a or OsPLGG1b knocked out were obtained. The phenotypes of osplgg1a-GOC plants were similar to osplgg1a, both of which showed stunted growth. Compared with GOC rice, the net photosynthetic rate of osplgg1b-GOC plants increased, indicating that OsPLGG1b mutation was conducive to the retention of chloroplast glycolate, increased the metabolic flux through the GOC bypass, reduced the photorespiration metabolism of plants, and further increased the CO₂ concentration in chloroplasts. Conclusion Compared with GOC rice, the net photosynthetic rate and stomatal conductance of osplgg1b-GOC plants were increased, suggesting that the elimination of OsPLGG1b could be used to optimize the photorespiratory metabolic pathway.
- OsPLGG1,
- photorespiration,
- GOC metabolic pathway,
- glycolate/glycerate transporter,
- rice

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References(34)

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