Diversity and Network Structure Analysis of Culturable Bacteria in Sporophore Site Soil of <i>Russula griseocarnosa</i><italic/>

WANG Yinghao; MAN Jiayin; ZHANG Taoxiang; LIAN Chunlan

doi:10.19303/j.issn.1008-0384.2022.004.014

Volume 37 Issue 4

Apr. 2022

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Fujian Journal of Agricultural Sciences > 2022 > 37(4): 529-537. > DOI: 10.19303/j.issn.1008-0384.2022.004.014

WANG Y H, MAN J Y, ZHANG T X, et al. Diversity and Network Structure Analysis of Culturable Bacteria in Sporophore Site Soil of Russula griseocarnosa [J]. Fujian Journal of Agricultural Sciences，2022，37（4）：529−537. DOI: 10.19303/j.issn.1008-0384.2022.004.014

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Diversity and Network Structure Analysis of Culturable Bacteria in Sporophore Site Soil of Russula griseocarnosa

WANG Yinghao^{1, 2,},
MAN Jiayin^{1, 2},
ZHANG Taoxiang^{1, 2, ,},
LIAN Chunlan³

1.
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
International Joint Laboratory of Forest Symbiology, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
3.
Asian Natural Environmental Science Center, The University of Tokyo, 1-1-8 Midori-cho, Nishitokyo, Tokyo 188-0002, Japan

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Received Date: March 07, 2022
Revised Date: March 27, 2022
Available Online: April 23, 2022

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Abstract

Abstract

Objective The diversity of culturable bacteria and their inter-species interactions were explored by isolating and culturing sporophore site soil bacteria of R. griseocarnosa. It provides insights into the mycorrhizal helper bacteria which may promote the growth of the mycelium and the formation and differentiation of sporophore of R. griseocarnosa.
Methods The bacteria in the sporophore site soil of R. griseocarnosa were isolated and purified by traditional methods of separation and culture using LB and ISP₂ media, and the species of the isolated strains was preliminarily identified by 16S rRNA gene sequence analysis. Interactions between culturable bacterial genera were analyzed using the Bipartite package in R.
Results A total of 128 strains of bacteria were isolated from 6 sporophore site soils of R. griseocarnosa, which belonged to 34 OTUs (16 genera of 3 phyla). The three genera with the highest abundance were Bacillus , Burkholderia and Streptomyces. Genus-level-Sample network analysis showed that the distribution of culturable bacteria was randomly nested. Bacillus and Burkholderia are important groups in the community, which have more Effective Partners, Closeness and high Species Strength in the bacterial community.
Conclusion There are abundant bacterial resources in the sporophore site soil of R. griseocarnosa. Combining the analysis of bacterial abundance and network structure can predict the interaction between bacteria more clearly.
- Russula griseocarnosa,
- rhizosphere soil,
- culturable bacteria,
- network structure

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Diversity and Network Structure Analysis of Culturable Bacteria in Sporophore Site Soil of Russula griseocarnosa

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