A Preliminary Study on Influenza Virus-induced Interferon Regulating Mechanism of SOCS3

PENG Benqun; HU Jingyun; MAO Yanan; WANG Shulin; WANG Jiajun; CHEN Mengying; YOU Dongxue; WANG Song

doi:10.19303/j.issn.1008-0384.2024.02.002

Volume 39 Issue 2

Feb. 2024

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Fujian Journal of Agricultural Sciences > 2024 > 39(2): 131-136. > DOI: 10.19303/j.issn.1008-0384.2024.02.002

PENG B Q, HU J Y, MAO Y N, et al. A Preliminary Study on Influenza Virus-induced Interferon Regulating Mechanism of SOCS3 [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：131−136. DOI: 10.19303/j.issn.1008-0384.2024.02.002

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A Preliminary Study on Influenza Virus-induced Interferon Regulating Mechanism of SOCS3

PENG Benqun^{1, 2, 3,},
HU Jingyun^{1, 2, 3},
MAO Yanan^{1, 2},
WANG Shulin^{1, 2},
WANG Jiajun^{1, 3},
CHEN Mengying^{1, 3},
YOU Dongxue^{1, 3},
WANG Song^{1, 2, 3, ,}

1.
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, Fuzhou, Fujian 350002, China
3.
Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, Fuzhou, Fujian 350002, China

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Received Date: January 02, 2024
Revised Date: January 28, 2024
Available Online: March 27, 2024

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Abstract

Abstract

Objective Regulatory function of the suppressor of cytokine signaling 3 (SOCS3) in the interferon signaling pathway during an influenza virus infection on cells was studied.
Method A549 cell lines were constructed with SOCS3 overexpression by a lentivirus infection and knockdown by siRNA technology. Along with control, they were infected with the influenza virus and sampled at times to assess the expressions and activation of crucial molecules within the interferon signaling pathway using RT-PCR and western blot analysis.
Result Decreasing expressions of type I interferon IFN-β and type III interferons IL-28 and IL-29 were observed following the SOCS3 overexpression in cells. Conversely, the SOCS3 knockdown raised the expressions of IFN-β, IL-28, and IL-29. SOCS3 overexpression also suppressed the expressions of the interferon regulatory factor IRF7 as well as those of the pattern recognition receptors RIG-I, MDA5, and TLR3 responsible for detecting influenza virus RNA. The virus induced SOCS3 overexpression inhibited, but the knockdown enhanced, the STAT1 phosphorylation.
Conclusion An influenza virus invasion on cells induced SOCS3 to recognize the receptors and regulate the mRNA expression inhibiting the type I and III interferon productions and STAT1 activation resulting in a blockage on the interferon signal transmission.
- Influenza virus,
- SOCS3,
- interferon,
- innate immunity,
- STAT1

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References

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